This laboratory course trains students in microbial isolation from diverse sources, including environmental, food, industrial, and clinical surrogates. Students will learn DNA extraction, PCR, qPCR, and digital PCR methods for microbial detection, identification, and quantification. Emphasis is placed on assay setup, troubleshooting, and analysis of data. Graduate students will additionally design and defend experimental protocols in the context of errors, ethics, and translational applications. Prereq: MICR 350L. Dual-listing: MICR 667.